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Results To analyze CTSL functions in epithelial tissue we expressed the mouse CTSL cDNA under the control of the human keratin 14 K14 promoter in transgenic mice.
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These proteases belong to a variety of distinct protease families, i.
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C Western blot for detection of EGF-receptor EGFR on keratinocytes and D quantitative RT-PCR real-time for quantification of EGF-receptor and GAPDH mRNA in absence of exogenous EGF.
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EGF is to a large extent synthesized by the submaxillary glands, but neither by fibroblasts nor by keratinocytes.
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The lysosomal cysteine protease cathepsin L regulates keratinocyte proliferation by control of growth factor recycling

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For preparation of primary dermal fibroblasts, the dermis was cut into small pieces and digested in 5 ml M-199 medium containing 0.
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The sum of radioactivity in all measured fractions obtained from a cell culture dish represents the total, i.
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4 M HEPES buffer and [ 3H]thymidine incorporation was determined by scintillation counting.
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63 kb] of the human growth hormone gene.
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